Scientific Reports of the National University of Life and Environmental Sciences of Ukraine

Detection of viruses of the rugose wood complex on vineyards of the Odessa region

N. Nikolaeva
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Abstract

Grape viruses cause great damage to vineyards in the south of Ukraine, especially viruses of the wood complex of the furrow complex (Rugose wood complex) (eng. - RWC). The aim of the research was to identify the presence of symptoms of viruses of this complex on vineyards in Odesa region and their identification. Methods. To conduct these studies, phytosanitary examination for the presence of wood furrow complex viruses was used, and real-time polymerase chain reaction (RT- -PCR) was used to identify viruses. Results. As a result of phytosanitary inspection of vineyards of Bolgrad, Izmiil and Ovidiopol districts of Odesa region, symptoms of viral damage to grape plants were revealed. For the first time, grape viruses were identified by a modified RT- PCR method, and diagnostic conditions were selected. Conclusions and prospects. As a result of phytosanitary inspection, grape viruses belonging to the furrow complex were found. The lesions of grape bushes by viruses of the wood furrow complex on the vineyards of the Bolgrad district of Odessa region were detected and identified. During the diagnosis, the PCR parameters were optimized, namely, the annealing temperature and magnesium concentration were tested. The obtained data will allow timely detection of viruses of the grape furrow complex, which can lead to a significant reduction in yield and prevent their spread

Keywords

RF-PCR, grapes, Grapevine virus A (GVA), Grapevine virus B (GVB), Rupestris stem pitting (RSPaV), LN 33 stem grooving

Suggested citation
Nikolaeva, N. (2022). Detection of viruses of the rugose wood complex on vineyards of the Odessa region. Scientific Reports of the National University of Life and Environmental Sciences of Ukraine, 18(3). https://doi.org/dopovidi2022.03.001
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