Scientific Reports of the National University of Life and Environmental Sciences of Ukraine

Validation of the method for determination of lead, cadmium, arsene in meat and meat products atomic-absorption spectrometry with electrothermal atom

S. Shulyak, К. Myagka, S. Tkachuk, О. Gaiduy, N. Mezhenskaya, I. Bardik
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Abstract

The toxicity of heavy metals poses risks to the health of both humans and animals based on their ability to accumulate in the body. The toxicity of the metal depends on the absorbed dose, route and duration of exposure. The timely determination of heavy metals in food, in accordance with EU-approved assessment methods, is an urgent task. The aim of the study was to validate the method of determination of lead, cadmium, arsenic in meat and meat products by atomic absorption spectrometry with electrothermal atomization. The Thermo Solaar atomic absorption spectrophotometer (USA) was used to determine arsenic and cadmium, and the Varian 240 G atomic absorption spectrophotometer (Australia) was used to determine lead. Nitric acid (Merk, Germany), certified standard solutions (Merk, Germany) according to the approved methods were used for mineralization of samples and preparation of background, calibration solutions. The validation characteristics of the method for determining lead, cadmium, arsenic by atomic absorption spectrometry with electrothermal atomization, namely: detection limit, quantitative limit, accuracy, correctness, convergence, reproducibility, selectivity, linearity. The accuracy, practicality and versatility of this method have been proven, which is confirmed by LOD and LOQ: for lead 0.31, 10.0 μg / kg, cadmium 0.3 and 5.0 μg / kg, arsenic 0.2 and 10.0 μg / kg. The results obtained and the validation data on accuracy and reproducibility are in line with the requirements of European legislation on the residual content of heavy metals in meat and meat products

Keywords

method validation, atomic absorption spectrometry, metals, meat and meat products

Suggested citation
Shulyak, S., Myagka, К., Tkachuk, S., Gaiduy, О., Mezhenskaya, N., & Bardik, I. (2021). Validation of the method for determination of lead, cadmium, arsene in meat and meat products atomic-absorption spectrometry with electrothermal atom. Scientific Reports of the National University of Life and Environmental Sciences of Ukraine, 17(5),129-141. https://doi.org/10.31548/dopovidi2021.05.012
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