Scientific Reports of the National University of Life and Environmental Sciences of Ukraine

Isolation of bovine coronavirus (BCOV) in cell cultures

А. Berezenko, V. Nedosekov, О. Godovskiy
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Abstract

One of the most common viruses in the world that causes disease in cattle is the bovine coronavirus (BCoV). This virus is the causative agent of respiratory and gastrointestinal infections in newborn calves, resulting in significant economic losses in both dairy and meat farming. Considering the complex epizootic situation with the coronaviruses in the world and partial antigenic affinity of BCoV with coronaviruses of other species of animals and humans, the isolation of new strains of coronaviruses, their identification and optimization of cultivation conditions becomes extremely important and relevant. The aim of our research was to determine the features of methods of isolation of bovine coronavirus and to select methods for its cultivation in cell culture in order to obtain the virus with the highest titers of infectious activity. The susceptibility of culture systems to the virus has been experimentally proven and it has been established that the use of the continious calf kidney cell culture line (MDBK) and the primary-trypsinized calf kidney culture (CK) allows to obtain a virus-containing suspension with a titer of infectious activity of 5.54 ± 0.16 lg TCD50/ml and 5.59 ± 0.14 lg TCD50/ml, respectively, which is promising for biotechnological developments. Further research is planned to be focused on studying of the obtained viral isolate for the subsequent development of tools for the diagnosis and immunoprophylaxis of coronavirus infection in veterinary medicine

Keywords

Coronaviridae, biological properties, bovine coronavirus, virus cultivation, cell culture, infectious activity

Suggested citation
Berezenko, А., Nedosekov, V., & Godovskiy, О. (2021). Isolation of bovine coronavirus (BCOV) in cell cultures. Scientific Reports of the National University of Life and Environmental Sciences of Ukraine, 17(4),5-16. https://doi.org/dopovidi2021.04.001
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