Influence of gibberellin on cultural and growth characteristics of vegetative mycelium of fungi strains of the genus Pleurotus during cultivation on agarized nutrient media

O. Kuznetsova
Abstract

The article discusses the use of a growth stimulator of gibberellin for the possible management of the development of vegetative mycelium of mushrooms, which are very little represented on the consumer market of Ukraine. The aim of this experiment was to study the nature of the influence of gibberellin on the cultural and growth characteristics of the vegetative mycelium of strains of the genus Pleurotus, which are classified as medium- and slow-growing, on agar nutrient media. The objects of the study were industrial strains of Pleurotus pulmonarius (Fr.) Quél. (strain IBK-230) (medium-growing), Pleurotus eryngii (DC.) Quél. (strains IBK-2011 and IBK-1972) (slow-growing) obtained from the Collection of cap mushrooms of the Institute of Botany named after N.G. Kholodny NAS of Ukraine. Surface cultivation was carried out in Petri dishes on agar nutrient media: corn agar, crushed sunflower husk agar, glucose-ammonium and glucose-asparagine agars with different concentrations of gibberellin (1, 10, 50, 100 mg / dm3). A decrease of the lag-phase of mycelium growth on media with gibberellin on average from 8 to 18%, an increase of the average radial mycelium growth rate from 16.8 to 57.9% for P. pulmonarius, and from 10.5 to 60% for strains P. eryngii, increasing the phenoloxidase activity of the mycelium was found. The most effective concentrations of gibberellin were found to be 10 and 50 mg / dm3, which confirms the data of other researchers established for Pleurotus ostreatus

Keywords

Pleurotus pulmonarius, Pleurotus eryngii, gibberellin, lag-phase, average radial mycelium growth rate, phenoloxidase

Suggested citation
Kuznetsova, O. (2020). Influence of gibberellin on cultural and growth characteristics of vegetative mycelium of fungi strains of the genus Pleurotus during cultivation on agarized nutrient media. Scientific Reports of the National University of Life and Environmental Sciences of Ukraine, 16(6). https://doi.org/10.31548/dopovidi2020.06.004
References
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