Scientific Reports of the National University of Life and Environmental Sciences of Ukraine

The degree of dominance of resistance trait against pathogens in F1 winter wheat

Liudmyla Murashko, Vira Kyrylenko, Oleksandr Humeniuk, Yuliia Suddenko, Nataliia Novytska
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Abstract

One of the most effective and at the same time environmentally friendly measures of integrated protection of wheat against diseases is the development of resistant varieties through breeding adapted to specific agroclimatic conditions. The purpose of the present study was the phytopathological evaluation of winter wheat collection samples on artificial infection backgrounds in the field and to determine the dominance of resistance of F₁ hybrids to Puccinia recondita and Tilletia caries. The study was conducted during 2023, 2024 in a selection crop rotation on natural and artificial infection backgrounds and in the laboratory of the winter wheat breeding laboratory of the V.M. Remeslo Myronivka Institute of Wheat of the National Academy of Agrarian Sciences of Ukraine according to generally accepted methods. 189 collection numbers of winter wheat were studied against artificial infectious backgrounds of brown rust and stinking smut. The study found that the varieties protected by resistance genes Arthur 71 (Lr9), McNair 2203 (Lr9), Agrus (Lr19), Century (Lr24+ Lr42), TAM-200 (Lr24+ Lr43), V1275 (Lr19), VR89Bo22 (Lr19) were immune to the pathogen Puccinia recondita; immunity to Tilletia caries was reflected by winter wheat varieties Rada (SVK), Famulus (DEU), SHARK/F4. 105W21 (USA), Reia (UKR), Ekspromt, Erythrospermum 24210, and Line 46 (UKR). When studying the composition of the population of Puccinia recondita, it was found that the average efficiency was shown by genes Lr10, Lr13, Lr25, and Lr34 (gene of age resistance) at the 5-10% degree of damage to the lines. The genes Lr9 and Lr19 stably maintained strong resistance and continued to be highly effective. In terms of resistance to Puccinia recondita, superdominance (heterosis) was found in five (14.72% of the total number of hybrid combinations) hybrid combinations Beres/Blueboy II, Beres/TAM-200, Matuo/TAM-200, Tobarzo/TAM-200, Matuo/Blueboy II. It was noted that the local population of the pathogen Tilletia caries is avirulent or slightly virulent to resistance genes: Bt6, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21. The study of the genetics of resistance to this disease showed that among F1 hybrid combinations, 41.63% showed partial positive dominance (PPD). No overdominance to the pathogen of stinking smut was found

Keywords

brown rust, resistance gene, variety, line, stinking smut of wheat, background

Suggested citation
Murashko, L., Kyrylenko, V., Humeniuk, O., Suddenko, Yu., & Novytska, N. (2025). The degree of dominance of resistance trait against pathogens in F1 winter wheat. Scientific Reports of the National University of Life and Environmental Sciences of Ukraine, 21(2),91-105. https://doi.org/10.31548/dopovidi/2.2025.91
References
  1. Alekseienko, E.V., & Kirchuk, E.I. (2022). Breeding value of donors of soft winter wheat resistance to brown rust in the south of Ukraine. Agrarian Innovations, 15, 78-82. doi: 10.32848/agrar.innov.2022.15.12.
  2. Babayants, L.T., Babayants, O.V., Baranovskaya, V.L., & Dubinina, L.A. (2006). Tilletia caries and resistance of wheat to this pathogen in Ukraine. Proceedings of the 15th biennial workshop on the smut fungi (pp. 33-36). Prague, Czech Republic.
  3. Babayants, O.V., & Babayants, L.T. (2014). Fundamentals of breeding and methodology of assessments of wheat resistance to pathogens. Odesa: WMW. doi: 10.33730/2077-4893.1.2019.163261.
  4. Bakhshi, T., Mehrabi, R., Sarbarzeh, M.A., Türkoğlu, A., & Haliloğlu, K. (2024). Screening diverse wheat genotypes for leaf rust resistance. Genetic Resources and Crop Evolution. doi: 10.1007/s10722-024-02285-9.
  5. Barabolya, O.V., & Borovko, S.V. (2024). Preservation of winter wheat yield-protection against smut diseases. In V international scientific and practical internet conference “Modern aspects and technologies in plant protection” (pp. 96-97). Poltava: Poltava State Agrarian University.
  6. Bashlay, A.G., & Vlasenko, V.A. (2020). Response of winter wheat plants to phytopathogens under conditions of biologisation of agriculture. Bulletin of Sumy National Agrarian University. The Series: Agronomy and Biology, 39(1), 3-13. doi: 10.32782/agrobio.2020.1.1.
  7. Beil, G.M., & Atkins, R.E. (1963). Inheritance of quantitative characters in grain sorghum. (Master’s thesis, Iowa State University, Ames, USA).
  8. Convention on Biological Diversity. (1992, June). Retrieved from https://zakon.rada.gov.ua/laws/show/995_030#Text.
  9. Datta, D., Prashar, M., & Bhardwaj, S.C. (2006). Pyramiding of leaf rust resistance genes Lr9 and Lr24 through molecular marker assisted selection in wheat (Triticum aestivum L.). Indian Journal of Genetics and Plant Breeding, 66(4), 332-334.
  10. Datta, D., Prashar, M., & Bhardwaj, S.C. (2007). Validation and incorporation of leaf rust resistance genes Lr9, Lr19, Lr24 and Lr26 through molecular markers in wheat (Triticum aestivum L.). Indian Journal of Genetics and Plant Breeding, 67(1), 7-11.
  11. Demidov, O.A., Kyrylenko, V.V., Gumenyuk, O.V., Murashko, L.A., Los, R.M., Suddenko, Yu.M., Mukha, T.I., Blyznyuk, B.V., & Dubovyk, N.S. (2023). Methodological approaches to the creation of breeding material of soft winter wheat resistant to fusarium graminearum schwabe in the conditions of the central Forest-Steppe of Ukraine. Kyiv: Komprint.
  12. Griffing, B. (1950). Analysis of quantitative gene action by constant parent regression and related techniques. Genetics, 35(3), 303-321. doi: 10.1093/genetics/35.3.303.
  13. Hanzalova, A., Šliková, S., Hudcovicová, M., Klčová, L., & Gregová, E. (2022). Virulence of wheat leaf rust (Puccinia triticina Eriks.) and Lr resistance genes in wheat cultivars in the Slovak Republic in the years 2016-2019. Cereal Research Communications, 50(2), 281-286. doi: 10.1007/s42976-021-00169-7.
  14. Kayim, M., Nawaz, H., & Alsalmo, A. (2022). Fungal diseases of wheat. London: IntechOpen. doi: 10.5772/intechopen.102661.
  15. Kolmer, J. (2013). Leaf rust of wheat: Pathogen biology, variation and host resistance. Forests, 4(1), 70-84. doi: 10.3390/f4010070.
  16. Krępski, T., et al. (2024). Leaf rust (Puccinia recondita f. sp. secalis) triggers substantial changes in rye (Secale cereale L.) at the transcriptome and metabolome levels. BMC Plant Biology, 24(1), article number 107. doi: 10.1186/s12870-024-04726-0.
  17. Liatukas, Ž., & Ruzgas, V. (2008). Resistance genes and sources for the control of wheat common bunt (Tilletia tritici (DC.) Tul.). Biologija, 54(4), 274-278. doi: 10.2478/v10054-008-0056-y.
  18. Lisova, H. (2023). Effectiveness of known wheat resistance genes Triticum aestivum L. to Puccinia triticina Eriks. leaf rust of wheat in 2019-2020. NaUKMA Research Papers. Biology and Ecology, 6, 17-26. doi: 10.18523/2617-4529.2023.6.17-26.
  19. Lunzer, M., Dumalasová, V., Pfatrisch, K., Buerstmayr, H., & Grausgruber, H. (2023). Common bunt in organic wheat: Unravelling infection characteristics relevant for resistance breeding. Frontiers in Plant Science, 14, article number 1264458. doi: 10.3389/fpls.2023.1264458.
  20. Markovska, O.E., Dudchenko, V.V., Grechyshkina, T.A., & Stetsenko, I.I. (2021). Development and spread of brown leaf rust of winter wheat depending on meteorological conditions, varietal composition and methods of protection. Tavriiskyi Naukovyi Visnyk, 117, 109-117.
  21. Mourad, A.M., Morgounov, A., Baenziger, P.S., & Esmail, S.M. (2022). Genetic variation in common bunt resistance in synthetic hexaploid wheat. Plants, 12(1), article number 2. doi: 10.3390/plants12010002.
  22. Mukha, T.I., & Murashko, L.A. (2019). Resistance of winter bread wheat collection nursery accessions to Fusarium ear blight and a group of diseases. Myronivskyi Vestnik, 9, 53-58. doi: 10.31073/mvis201909-07.
  23. Murashko, L.A., Mukha, T.I., Humeniuk, O.V., Novytska, N.V., & Martynov, O.M. (2022). Assessment of resistance of winter wheat varieties of Ukrainian breeding centres against diseases on artificial infectious backgrounds of their pathogens. Agrarian Innovations, 13, 209-214.
  24. Pal, D., et al. (2024). Identification of rust resistance genes in wheat (Triticum aestivum L.) using molecular markers and host–pathogen interaction tests. Journal of Phytopathology, 172(5), article number e13417. doi: 10.1111/jph.13417.
  25. Palamarchuk, A., Rubezhniak, I., & Chaika, V. (2018). Spread of winter wheat diseases in Ukraine. Biological Resources and Nature Management, 10(3-4), 64-71. doi: 10.31548/bio2018.03.008.
  26. Ritzer, E., Ehn, M., Oberforster, M., & Buerstmayr, H. (2021). Comparison of pathogenicity of Austrian isolates of Tilletia caries on common wheat (Triticum aestivum). In Plant breeding for the “Green Deal” (pp. 75-76). Vienna: Association of Plant Breeders and Seed Traders of Austria. doi: 10.5281/zenodo.5667799.
  27. Samoilik, M.O. (2024). Breeding value of the source material of soft winter wheat created by hybridisation of different ecotypes. (PhD thesis, Bila Tserkva National Agrarian University, Bila Tserkva, Ukraine).
  28. Sauliak, N.I., Traskovetska, V.A., Vasyliev, O.A., Bushulian, M.A., Rudenko, V.A., & Tsapenko, V.M. (2024). Resistance of soft winter wheat varieties against pathogens of major leaf blight diseases in the South of Ukraine. Plant Varieties Studying and Protection, 20(2), 84-89. doi: 10.21498/2518-1017.20.2.2024.304104.
  29. Topko, R., Vologdina, G., Humenyuk, O., & Kovalyshyna, H. (2021). Spectral assessment of winter wheat varieties and breeding lines in the autumn period. Plant and Soil Science, 12(2), 29-36. doi: 10.31548/agr2021.02.0029.
  30. Tribel, S.O., Hetman, M.V., Strigun, O.O., Kovalyshina, G.M., & Andriushchenko, A.V. (2010). Methodology for assessing the resistance of wheat varieties against pests and pathogens. Kyiv: Kolobig.
  31. Xu, S., et al. (2018). Evaluation of selected advanced spring wheat germplasm lines in Eastern Canada. Sustainable Agriculture Research, 7(3), 63-70. doi: 10.5539/sar.v7n3p63.