Biotesting of the anthropogenic impact on areas of the r. Ros and r. Protoka on the viability of carp fish eggs and larvae

Yu. Khudiiash, O. Potrokhov, O. Zinkovskyi, O. Vodianitskyi, K. Kofonov, Yu. Krasiuk
Abstract

Therefore, a special role in the toxic effect on the viability of fish larvae is assigned to the presence of inorganic nitrogen in the water. An excessive intake of nitrogen compounds, especially ammonium and nitrite, significantly reduces the viability of carp larvae that have left the shell. It was established that a clearer indicator of the toxicity of the environment is the survival rate of both species of fish, which are at the early post-embryonic stage of development The results of research into the hydrochemical state of experimental reservoirs, which fully correspond to the nature of pollution, are presented. A significant increase in the concentration of ammonium nitrogen in the water of the Ros River in the area of the villages Horodishche, Pylypcha and of the Protoka River near the village of Pishchana was noted by compared to the Ros River near the Oleksandria Arboretum. There is also an excess of the concentration of nitrite ions in the water in the Protoka river near the village Pishchana compared to other experimental sites, which indicates fresh pollution of the reservoir. In the water of the Ros river in the area of the villages Horodishche, Pylypcha and in the water of the Protoka River near the village Pishchana content of nitrate ions was higher compared to the Ros River (near the Oleksandria Arboretum), which is a consequence of the constant inflow of leachate from agricultural lands, which are adjacent territories to these areas. The level of pollution of some areas of the Ros River and the Protoka River was studied in terms of survival (viability) of developing eggs and larvae of carp and white carp. The analysis of the results of biotesting of the degree of toxicity of water from these reservoirs showed that the viability of eggs in water from areas located near the villages Pylypcha and Pishchana was slightly larger than that of the eggs, which was incubated in water from the area near the village Horodishche and Arboretum of Oleksandria. The decrease in the survival rate of fish embryos (the village of Gorodishche and Oleksandria Arboretum) is indirectly related to the higher concentration of phosphate ions in the water sampled at these sites. Under such conditions, an increase in the number of bacterioplankton is possible, which disrupts the oxygen regime in the environment and leads to negative consequences. In the most polluted water near the villages Pishchana and Horodishche, the survival rate of carp larvae was lower than in the water near the Oleksandria Arboretum and the village Pylypcha. This indicates that in the larval stages of development, fish are most vulnerable to a toxic environment. It was noted that the concentration of phosphorus and phosphates in the section of the Protoka River near the village Pishchana was minimal compared to other experimental sampling points.

Keywords

biotesting, survival, fish eggs and larvae, ammonium nitrogen, nitrites, nitrates, phosphates, environmental toxicity

Suggested citation
Khudiiash, Yu., Potrokhov, O., Zinkovskyi, O., Vodianitskyi, O., Kofonov, K., & Krasiuk, Yu. (2023). Biotesting of the anthropogenic impact on areas of the r. Ros and r. Protoka on the viability of carp fish eggs and larvae. Scientific Reports of the National University of Life and Environmental Sciences of Ukraine, 19(6). https://doi.org/10.31548/dopovidi6(106).2023.003
References

[1] Levkivskyi, S.S., & Padun, M.M. (2006). Rational use and protection of water resources. Kyiv: Lybid.

[2] Khilchevskyi, V.K., Zabokrytska, M.R., & Kravchynskyi, R.L. (2016). Ecological standardization and prevention of waste impact on the environment. Kyiv: VPTs Kyivskyi Universytet.

[3] Vasenko, O.H., et al. (2015). Integral and complex assessments of the environmental state. Kharkiv: NUHZU.

[4] Petrenko, O.V., & Pavlenko, V.O. (Eds.). (2015). Environmental monitoring. Kyiv: Kyivskyi Universytet.

[5] Matsniev, A.I., et al. (2000). Monitoring and engineering methods of environmental protection: Textbook. Rivne: VAT Rivnenska Drukarnia.

[6] EU Water Framework Directive 2000/60/EC. (2000). Official Journal of the European Communities, 22.12.2000, L 327/1.

[7] Chukhrii, Yu.P. (2014). Bioindication. biotesting. biomonitoring: Lecture notes. Odesa: ONAKHT.

[8] Lysytsia, A.V. (2018). Bioindication and biotesting of contaminated territories: Methodological recommendations for self-study. Rivne: Doka-Tsentr.

[9] Krainiukov, O.M., Striian, K.O., & Krainiukov, O.O. (2018). Establishing metrological characteristics of biotesting methodology for determining acute lethal toxicity of water on fish Brachydanio rerio Hamilton-Buchanan. Biological Sciences Young Scientist, 9(61), 279-281.

[10] Khilchevskyi, V.K. (Ed.). (2009). Hydroecological state of the Ros River basin. Kyiv: Nika-Tsentr.

[11] Shevchenko, P.H., et al. (2022). Practical course in ichthyology (general and special). Kherson: Oldi-Plius.

[12] Hayatsu, M., Tago, K., & Saito, M. (2008). Various players in the nitrogen cycle: Diversity and functions of the microorganisms involved in nitrification and denitrification. Soil Science and Plant Nutrition, 54.

[13] Sadovska, I.B. (2010). Analysis and calculation of the nitrification process in wastewater treatment. Environmental Safety and Nature Management: Collection of Scientific Papers, 6, 129-139.

[14] Henze, M., Harremoes, P., Jansen, G., & Arwin, E. (2002). Wastewater treatment. Berlin, New York: Springer.

[15] Klymenko, M.O. (2006). Environmental monitoring. Kyiv: Akademiia.

[16] Avramenko, N.I. (2013). Types of algae in the Vorskla River causing water "blooming." Bulletin of Poltava State Agrarian Academy, 1, 158-162.