Scientific Reports of the National University of Life and Environmental Sciences of Ukraine

The influence of seed-dressings on the formation and functioning of symbiotic soybean systems with fungicide-resistant rhizobia

К. Kukol, N. Vorobey, P. Pukhtaievych, S. Kots
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Abstract

The application of bacterial fertilizers, prepared on the basis of active, competitive strains of nitrogen-fixing microorganisms and fungicidal dressings for pre-sowing seed treatment in soybean cultivation technologies is characterized by high biological and economic efficiency. Therefore, it is important to find such combination of chemicals and microbial preparations, for the complex use of which will be maintained their main purpose. The aim of the work was to investigate the formation and functioning of symbiotic soybean systems with fungicide-resistant nodule bacteria Bradyrhizobium japonicum PC09 and B144 under the influence of Standak Top and Fever seed dressings. Differences in the effect of chemical plant protection products on the formation of symbiotic systems of soybean variety Almaz and nitrogen-fixing microorganisms have been established. A decrease by 9.2‒24.1% in the number of nodules formed on the roots during the soybean growing season with the combined use of Fever with rhizobia bacterization of PC09 strain was revealed. At inoculation the seed with Tn5- mutant B144, use of the same preparation, the decrease of this indicator compared to control plants was 6.5‒32.8%. An increase in the weight of root nodules by 10.3‒36% was found under the effect of Standak Top and bacterization by both strains of rhizobia. There was a decrease in the intensity of N2 assimilation compared to control plants by 24.2 and 42.3% in the stage of three true leaves with the combined use of Fever with inoculation and a gradual decrease of the toxic effect of pesticides on the functioning of symbiotic systems in the budding-flowering stage. At shared treatment of seeds with rhizobia strains B144 and PC09 and Standak Top revealed an increase in nitrogen fixation intensity by 6.2‒25 % in the stage of three true leaves, 12.5‒58% in the stage of budding-beginning of flowering and by 28.1‒42.3% in the stage of bean formation. Maintaining high level of nitrogenase activity of legume-rhizobial symbiosis, formed with the participation of pesticide-resistant nodule bacteria with using of seed dressings, will provide plants with available forms of nitrogen, protect sown seeds from soil and seed infections and create conditions for grain production

Keywords

soybeans, fungicides, inoculation, seed dressing, number and weight of nodules, nitrogen-fixing activity

Suggested citation
Kukol, К., Vorobey, N., Pukhtaievych, P., & Kots, S. (2022). The influence of seed-dressings on the formation and functioning of symbiotic soybean systems with fungicide-resistant rhizobia. Scientific Reports of the National University of Life and Environmental Sciences of Ukraine, 18(1). https://doi.org/10.31548/dopovidi2022.01.003
References
  1. Kulkarni, K.P., Tayade, R., Asekova, S., Song, J.T., Shannon, J.G., & Lee, J.D. (2018). Harnessing the potential of forage legumes, alfalfa, soybean, and cowpea for sustainable agriculture and global food security. Frontiers in Plant Science, 9, article number 1314. https://doi.org/10.3389/fpls.2018.01314.
  2. Kots, S.Ya., Vorobey, N.A., & Kyrychenko, O.V. (2016). Microbiological preparations for agriculture. Institute of Plant Physiology and Genetics NAS of Ukraine. Kyiv: Logos.
  3. Pospielova, G.D., Kovalenko, N.P., Nechyporenko, N.I., & Kocherga, V.Ya. (2020). Influence of agroclimatic factors on the development of common soybean diseases. Bulletin of Agricultural Science of the Black Sea Region, 3(107), 45-52. https://doi.org/10.31521/2313-092X/2020-3(107).
  4. Munkvold, G.P., Watrin, C., Scheller, M., Zeun, R., & Olaya, G. (2014). Benefits of chemical seed treatments on crop yield and quality. In M. Gullino & G. Munkvold (Eds.), Global perspectives on the health of seeds and plant propagation material. Plant pathology in the 21st century (Vol. 6). Springer. https://doi.org/10.1007/978-94-017-9389-6_7.
  5. Trybel, S.O., & Strygun, O.O. (2013). Protection of plants – real trend of increasing crop production. Plant Protection and Quarantine, 59, 324-336.
  6. Sadovnikova, L.K., Orlov, D.S., & Lozanovskaya, I.N. (2006). Ecology and environmental protection in case of chemical pollution. Moscow: Vyisshaya shkola.
  7. Safronava, G.V., Sukhovitskaya, L.A., & Karalenak, N.V. (2007). The effect of inocula and pesticides on development of legume-rhizobial symbiosis and productivity of grain-legume crops. Agricultural Microbiology, 5, 61-73.
  8. Schnelle, M.A., & Hensley, D.L. (1990). Effects of pesticides upon nitrogen fixation and nodulation by dry bean. Pest Management Science, 28(1), 83-88. https://doi.org/10.1002/ps.2780280110.
  9. Borzenkova, G.A. (2014). Optimization of the technology of preseeding treatment and possibility of its combination with inoculation for protection of soya against contamination with seed infection. Grain Legumes and Cereal Crops, 1(9), 22-30.
  10. Mikolaievsky, V.P., Sergienko, V.G., & Tytova, L.V. (2016). Diseases development and productivity of soybean at the seeds treatment by microbial formulations. Agrobiology, 2, 96-103.
  11. Zilli, J.Е., Ribeiro, K.G., Campo, R.J., & Hungria, M. (2009). Influence of fungicide seed treatment on soybean nodulation and grain yield. Revista Brasileira de Ciência do Solo, 33(4), 917-923. https://doi.org/10.1590/S0100-06832009000400016.
  12. Biliavska, L.H., & Biliavskyi, Yu.V. (2007). New early-style soya variety Almaz. Bulletin of Poltava State Agrarian Academy, 2, 56-57.
  13. Yaschuk, V.U., Ivanov, D.V., Krivosheya, R.M., Tsibulnyak, Yu.O., & Koretskiy, A.P. (2018). The list of pesticides and agrochemicals permitted for use in Ukraine. Kyiv: Yunivest Media.
  14. Vorobey, N.А., Kukol, K.P., & Kots, S.Ya. (2020). Fungicides toxicity assessment on Bradyrhizobium japonicum nodule bacteria in pure culture. Microbiological Journal, 82(3), 45-54. https://doi.org/10.15407/microbiolj82.03.045.
  15. Grodzinskij, A.M., & Grodzinskij, D.M. (1964). A short guide to plant physiology. Kiev: Nauk. Dumka.
  16. Hardy, R.W.F., Holsten, R.D., Jackson, E.K., & Burns, R.C. (1968). The acetylene-ethylene assay for N2 fixation: laboratory and field evaluation. Plant Physiology, 43(8), 1185-1207. https://doi.org/10.1104/pp.43.8.1185.
  17. Kaur, S., & Kaur, G. (2018). Morphological and physiological aspects of symbiotic plant–microbe interactions and their significance. In Root Biology (pp. 367-407). Springer. https://doi.org/10.1007/978-3-319-75910-4_15.
  18. Mamenko, T.P., Kots, S.Ya., & Khomenko, Y.O. (2020). The intensity of ethylene release by soybean plants under the influence of fungicides in the early stages of legume-rhizobial symbiosis. Regulatory Mechanisms in Biosystems, 11(1), 98-104. https://doi.org/10.15421/022014.
  19. Yakimenko, M.V., Begun, S.A., & Sorokina, A.I. (2016). Compatibility of collection strains of soy Rhizobia with fungicides and growth stimulants. Far Eastern Agrarian Bulletin, 2(38), 38-41.
  20. Kirichenko, Е., Pavlyshche, А., Omelchuk, S., Zhemojda, A., & Коts, S. (2020). Physiological aspects of the response of soybean-rhizobial symbiosis to the action of fungicides Standak Top and Fever. Ştiinţa Agricolă, 2, 59-72. https://doi.org/10.5281/zenodo.4320984.
  21. Romero-Perdomo, F.A., Camelo, M., & Bonilla, R. (2015). Response of Bradyrhizobium japonicum to alginate in presence of pelleted fungicides on soybean seeds. Revista U.D.C.A Actualidad & Divulgación Científica, 18(2), 359-364.