Scientific Reports of the National University of Life and Environmental Sciences of Ukraine

Research of microcystins as inhibitors of Phytophthora Infestans development

V. Nykyforov, О. Novokhatko, О. Maznitska, О. Sakun, S. Digtiar
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Abstract

Information from foreign literary sources regarding microcystins of blue-green algae is presented. The current state of the problem of reservoirs «blooming» and the significance of this phenomenon for humans are reflected. The research was carried out in two stages: laboratory and full-scale. A pure culture of Phytophthora infestans was isolated, on which further studies were carried out. Isolation of isolates was carried out on agar nutrient medium. Potassium permanganate and ethanol were selected from the available antiseptics. Field experiments were carried out on experimental lines of Solanum lycopersicum by diagnosing signs of late blight disease. The determination of the potential negative effect of tomato plants treated with a suspension of cyanobacteria was carried out by the method of biotesting using Achatina fulica as a test object. The effect of blue-green algae toxins – microcystins – on colonies Ph. infestans in vitro is described. Photometric observation of the decrease in the number of colonies with a time interval of three days is presented. The phytophtorostatic effect of microcystins has been established. Degradation of Ph. infestans the next day were fixed after treatment of the colony with a suspension of cyanobacteria. An inhibitory effect was revealed, almost to the complete disappearance of symptoms in plants partially affected by late blight and grown in vivo. It has been determined that plants treated with microcystin can be considered safe for further consumption; the death of model organisms has not been registered

Keywords

microcystin, toxicity, inhibitors, phytophthora, in vitro, biotesting, test object, in vivo, biosafety

Suggested citation
Nykyforov, V., Novokhatko, О., Maznitska, О., Sakun, О., & Digtiar, S. (2021). Research of microcystins as inhibitors of Phytophthora Infestans development. Scientific Reports of the National University of Life and Environmental Sciences of Ukraine, 17(5),5-17. https://doi.org/10.31548/dopovidi2021.05.001
References
  1. Lamour, K. (Ed.). (2013). Phytophthora. A Global Perspective. Wallingford: CAB International.
  2. Buratti, F., Testai, E., Vichi, S., Sacchi, A., Funari, E., Isidori, M., Parrone, D., Cangiano, M., Matthiessen, P., Arukwe, A., & Andreassen, I.K. (2013). The conjugation of microcystin-RR by human recombinant GSTs and hepatic cytosol. Toxicology Letters, 219, 231-238.
  3. Gutierrez-Praena, D., Jos, A., Pichardo, S., Moreno, I.M., & Cameán, A.M. (2013). Presence and bioaccumulation of microcystins and cylindrospermopsin in food and the effectiveness of some cooking techniques at decreasing their concentrations: A review. Food and Chemical Toxicology, 53, 139-152.
  4. Buratti, F., Manganelli, M., Vichi, S., Stefanelli, M., Scardala, S., Testai, E., & Funari, E. (2017). Cyanotoxins: Producing organisms, occurrence, toxicity, mechanism of action and human health toxicological risk evaluation. Archives of Toxicology, 91, 1049-1130.
  5. Sivonen, K., & Borner, T. (2008). Bioactive compounds produced by cyanobacteria. In A. Herrero & E. Flores (Eds.), The Cyanobacteria Molecular Biology, Genomics and Evolution (pp. 158-197). Norfolk, UK: Caiser Academic Press.
  6. Finking, R., & Marahiel, M.A. (2004). Biosynthesis of nonribosomal peptides. Annual Review of Microbiology, 58, 453-488.
  7. Sieber, S.A., & Marahiel, M.A. (2004). Molecular mechanisms underlying nonribosomal peptide synthesis: Approaches to new antibiotics. Chemical Reviews, 105, 715-738.
  8. Dittmann, E., Neilan, B.A., Erhard, M., von Dohren, H., & Borner, T. (2005). Insertional mutagenesis of a peptide synthetase gene that is responsible for hepatotoxin production in the cyanobacterium Microcystis aeruginosa PCC 7806. Molecular Microbiology, 26, 779-787.
  9. Rouhiainen, L., Vakkilainen, T., Siemer, B.L., Buikema, W., Haselkorn, R., & Sivonen, K. (2004). Genes coding for hepatotoxic heptapeptides (microcystins) in the cyanobacterium Anabaena strain 90. Applied and Environmental Microbiology, 70, 686-692.
  10. Pearson, L.A., Hisbergues, M., Borner, T., Dittmann, E., & Neilan, B.A. (2004). Inactivation of an ABC transporter gene, mcyH, results in loss of microcystin production in the cyanobacterium Microcystis aeruginosa PCC 7806. Applied and Environmental Microbiology, 70, 6370-6378.
  11. Dunn, A., Bruton, S., Clark, W., Grogan, H., O'Brien, P., Ryder, D., & Wedgwood, E. (2010). Population structure and resistance to mefenoxam of Phytophthora capsici in New York state. Plant Disease, 94, 1461-1468.
  12. Forster, H., Cummings, M., & Coffey, M. (2000). Phylogenetic relationships of Phytophthora species based in ribosomal ITS I DNA sequence analysis with emphasis on Waterhouse groups V and VI. Mycological Research, 104, 1055-1061.
  13. Nykyforov, V., Malovanyy, M., Kozlovska, T., Novokhatko, O., & Digtiar, S. (2016). The biotechnological ways of blue-green algae complex processing. Eastern-European Journal of Enterprise Technologies, 5/10(83), 11-18.
  14. Sakun, O.A., Shendryk, V.S., & Kovalenko, Ya.A. (2019). Study of the effect of microcystins for the prevention of late blight of tomatoes. Bulletin of Kremenchuk Mykhailo Ostrohradskyi National University, 5(118), 58-65.