Scientific Reports of the National University of Life and Environmental Sciences of Ukraine

The Grapevine Leaf Roll-Associated Virus (GLRaV) is a malicious virus of grape plants. The 9th serotypes of this virus have been established (GLRaV1-9). The most common are physiologically related 1st and 3rd serotypes. The spread of the disease, the causative agent of which is the leaf curling virus, occurs with infected planting material. Therefore, timely accurate diagnosis of the disease and identification of its pathogen is necessary. The aim of the study was to identify and identify the Grapevine Leaf Roll-Associated Virus of different serotypes in the vineyards of southern Ukraine. To achieve the goal, the following tasks were set: to investigate the degree of infestation of vineyards with the leaf curling virus; conduct serological and molecular biological diagnostics to identify GLRaV and compare the results of research using these methods. To perform this work, the following methods were used: ELISA (enzyme-linked immunosorbent assay), classical RT-PCR (reverse transcription polymerase chain reaction), and real-time RT-PCR. The study revealed of Grapevine Leaf Roll-Associated Virus of the 1st and 3rd serotypes in the vineyards of southern Ukrainе

Grapevine Leaf Roll-Associated Virus (GLRaV), ELISA, RT-PCR, vineyards, grape viral disease