Determining the effect of different aeration regimes on technological parameters of waste fermentation

Andrii Kachur, Oksana Pylypchuk
Abstract

The purpose of the study was to determine the optimal frequency of mechanical mixing of the substrate to intensify biothermal processes, stimulate microbiological activity, and increase the efficiency of bioconversion of organic matter. The study was performed under the conditions of an experimental farm using organic waste of plant origin and the biodestructor “Unikal-S”. The effect of aeration on the temperature regime and changes in moisture content of the compost mass was analysed during 150 days of fermentation. Different mechanical mixing regimes were used: once every 3, 5, 7, and 10 days. Compost temperature was monitored daily, and moisture content was determined once every 5 days according to applicable standards. The results demonstrate that regular aeration contributed to a more intensive course of biothermal processes in soybean waste and spoiled corn silage. The highest temperature values were recorded in the variants with mechanical mixing once every 3 days. The temperature reached 62°C in soybean waste and 66°C in spoiled corn silage, indicating active development of thermophilic microflora and intensive decomposition of organic matter. More frequent mixing ensured longer maintenance of the thermophilic phase of fermentation and contributed to a more effective decrease in substrate moisture. Aeration once every 3-5 days provided the best technological parameters of fermentation and intensified bioconversion of organic waste. The results can be used to improve plant waste composting technologies and increase the efficiency of fermentation processes in agriculture

Keywords

aeration; composting; soybean waste; biodestructor; compost temperature; moisture; bioconversion

Suggested citation
Kachur, A., & Pylypchuk, O. (2026). Determining the effect of different aeration regimes on technological parameters of waste fermentation. Scientific Reports of the National University of Life and Environmental Sciences of Ukraine, 22(3),199-212. https://doi.org/10.31548/dopovidi/3.2026.199
References
  1. Arriola, K.G., Kim, S.C., & Adesogan, A.T. (2011). Effect of applying inoculants with heterolactic or homolactic and heterolactic bacteria on the fermentation and quality of corn silage. Journal of Dairy Science, 94, 1511-1516. doi: 10.3168/jds.2010-3807.
  2. Asghar, A., Afzaal, M., Nosheen, F., Saeed, F., Nayik, G.A., Al-Farga, A., Alansari, W.S., Eskandrani, A.A., & Shamlan, G. (2022). Isolation and molecular characterization of processed soybean waste for the development of synbiotic yogurt. Fermentation, 8(11), article number 622. doi: 10.3390/ fermentation8110622.
  3. Atauzzaman, M., & Bari, Q.H. (2023). Effect of passive and forced aeration on composting of market solid waste. In Organic fertilizers – new advances and applications (pp. 149-162). London: IntechOpen. doi: 10.5772/intechopen.1001328.
  4. Chang, C.-T., Negi, S., Rani, A., Hu, A.H., Pan, S.-Y., & Kumar, S. (2022). Food waste and soybean curd residue composting by black soldier fly. Environmental Research, 214(1), article number 113792. doi: 10.1016/j.envres.2022.113792
  5. Costa, D.M., Carvalho, B.F., de Souza, V.C., Pereira, M.N., & da Silva Ávila, C.L. (2023). Particle size and storage length affect fermentation and ruminal degradation of rehydrated corn grain silage. Archives of Animal Nutrition, 77(3), 245-259. doi: 10.1080/1745039X.2023.2219177.
  6. DSTU OIML R 133:2019. Glass liquid-filled thermometers (OIML R 133:2002, IDT)(2019). Retrieved from https://online.budstandart.com/ua/catalog/doc-page.html?id_doc=89199.
  7. ISO 6496:1999. Animal feeding stuffs – determination of moisture and other volatile matter content. (1999). Retrieved from https://www.iso.org/standard/12871.html.
  8. Keng, Z.X., Tan, J.J.M., Phoon, B.L., Khoo, C.C., Khoiroh, I., Chong, S., Supramaniam, C., Singh, A., & Pan, G.-T. (2023). Aerated static pile composting for industrial biowastes: From engineering to microbiology. Bioengineering, 10(8), article number 938. doi: 10.3390/bioengineering10080938.
  9. Mitiohlo, L.V., Merzlov, S., & Merzlova, H. (2023b). Indicators of spoiled corn silage during its fermentation with different doses of biodestructor. Scientific Progress & Innovations, 26(3), 76-80. doi: 10.31210/spi2023.26.03.14.
  10. Mitiohlo, L.V., Merzlov, S.V., Merzlova, H.V., & Babenko, S.P. (2023a). The content of micro-elements in fermented corn silage and alfalfa sayage. Scientific and Technical Bulletin оf State Scientific Research Control Institute of Veterinary Medical Products and Fodder Additives аnd Institute of Animal Biology, 24(1), 88-97. doi: 10.36359/scivp.2023-24-1.13.
  11. Nasir, M., Rauf, R., Liaqat, I., Tuseef, M., Abbas, S., & Kordor, W.G. (2025). Utilization of food industry byproducts in functional foods. In S.H. Farooqi, K. Kholik & M.A. Zaman (Eds.), One health horizons: Integrating biodiversity, biosecurity, and sustainable practices (pp. 80-87). Faisalabad: Unique Scientific Publishers. doi: 10.47278/book.HH/2025.299.
  12. Panda, S., & Jain, M.S. (2025). A systematic review of prevalent soy waste management techniques. Renewable and Sustainable Energy Reviews, 212, article number 115305. doi: 10.1016/j.rser.2024.115305
  13. Patel, K.K., Baghel, S.S., Agrawal, S.B., Rai, H.K., Sahu, R.K., Singh, U., Shah, A.K., & Priya. (2025). Enriched vermicompost made through bio waste of soybean stover (dry matter) and fresh cow dung using earthworms (Eisenia fetida spp.). Asian Journal of Soil Science and Plant Nutrition, 11(1), 165-172. doi: 10.9734/AJSSPN/2025/v11i1471.
  14. Raut, M.P., Prince William, S.P.M., Bhattacharyya, J.K., Chakrabarti, T., & Devotta, S. (2008). Microbial dynamics and enzyme activities during rapid composting of municipal solid waste – a compost maturity analysis perspective. Bioresource Technology, 99(14), 6512-6519. doi: 10.1016/j. biortech.2007.11.030.
  15. Sánchez-Monedero, M.A., Cayuela, M.L., Roig, A., Jindo, K., Mondini, C., & Bolan, N.S. (2018). Role of biochar as an additive in organic waste composting. Bioresource Technology, 247, 1155-1164. doi: 10.1016/j.biortech.2017.09.193.
  16. Santi, G., Proietti, S., Moscatello, S., Stefanoni, W., & Battistelli, A. (2015). Anaerobic digestion of corn silage on a commercial scale: Differential utilization of its chemical constituents and characterization of the solid digestate. Biomass and Bioenergy, 83, 17-22. doi: 10.1016/j. biombioe.2015.08.018.
  17. Sundberg, C. (2005). Improving compost process efficiency by controlling aeration, temperature and pH. (Doctoral dissertation, Swedish University of Agricultural Sciences, Uppsala, Sweden). 
  18. Tang, Y., Lu, G., Zhao, H., Li, L., Liao, C., Wang, P., Zhang, Y., Zhang, M., Li, P., & Gou, W. (2025). Effect of distillery spent wash utilization on maize silage fermentation and quality. Animals, 15(21), article number 3146. doi: 10.3390/ani15213146.
  19. Usman, M., Li, Q., Luo, D., Xing, Y., & Dong, D. (2025). Valorization of soybean by-products for sustainable waste processing with health benefits. Journal of the Science of Food and Agriculture, 105, 5150-5162. doi: 10.1002/jsfa.13999.
  20. Volkogon V.V., M’iagka M.V., Dimova S.B., Derkach S.M., Pyrig O.V., & Lutsenko N.V. (2019). Influence of introduction of cellulosolitic microorganisms on microbiocenosis in conditions of a composting of a poultry excreta. Bulletin of Agricultural Science, 97(5), 53-64. doi: 10.31073/agrovisnyk201905-07.
  21. Waqas, M., Hashim, S., Humphries, U.W., Ahmad, S., Noor, R., Shoaib, M., Naseem, A., Hlaing, P.T., & Lin, H.A. (2023). Composting processes for agricultural waste management: A comprehensive review. Processes, 11(3), article number 731. doi: 10.3390/pr11030731.
  22. Wilkinson, J.M., Bolsen, K.K., & Lin, C.J. (2015). History of Silage. In D.R. Buxton, R.E. Muck & J.H. Harrison (Eds.), Silage science and technology (pp. 1-30). doi: 10.2134/agronmonogr42.c1.
  23. Zhou, P., Wu, G., Luo, X., Ma, Y., Guan, K., Pang, H., Tan, Z., Zhang, S., & Wang, L. (2025). Comprehensive evaluation of agricultural residues corn stover silage. Agriculture, 15(13), article number 1362. doi: 10.3390/agriculture15131362.