Formation and utilisation of alfalfa sward under the soil and climatic conditions of the Right-Bank Forest-Steppe

Nadiya Hetman, Borys Danyliuk, Lesia Burko
Abstract

The aim of the study was to determine the optimal combination of sowing rate and complex mineral fertilisers for the formation of a productive alfalfa (Medicago sativa L.) sward of the ‘Radoslava’ variety under the soil and climatic conditions of the Right-Bank Forest-Steppe. The experiment was conducted on grey forest soils during 2024-2025 using a three-factor design: sowing rates of 6.0 and 8.0 million viable seeds per hectare, two harvesting periods, and three levels of mineral nutrition. It was established that in the year of sowing (2024), the crop reached the beginning of flowering stage (BBCH code 61-62) after 69-70 days, with a daylight duration of 14:53-16:12 hours, and provided three cuts with a total green matter yield of up to 33.13 t/ha. The greatest plant height in the first cut was 66.1-66.3 cm at a sowing rate of 6.0 million/ha and maximum fertilisation (Background +K₆₀S₂₀Ca₃₀Mg₂₀), whereas in the third cut it decreased to 21.2-30.3 cm due to reduced daylight duration and increased temperature. On average across the treatments, plant height ranged from 36.00 to 42.93 cm, while leafiness ranged from 33.74 to 40.77%. In the second year (2025), across three cuts at the beginning of flowering stage (BBCH code 64), the average plant height was 61.4-61.8 cm, leafiness was 53.0-54.3%, and green matter yield reached 75.8 t/ha. The statistical significance of factor effects was confirmed by LSD₀.₀₅. It was determined that the optimal combination was a sowing rate of 6.0 million/ha with increased fertilisation (Background +K₆₀S₂₀Ca₃₀Mg₂₀), which ensured the highest total green matter yield with minimal risks of moisture deficiency under the unstable hydrothermal conditions of the Right-Bank Forest-Steppe

Keywords

sowing rate; mineral fertilisers; plant height; leafiness; green matter yield; mowing time

Suggested citation
Hetman, N., Danyliuk, B., & Burko, L. (2026). Formation and utilisation of alfalfa sward under the soil and climatic conditions of the Right-Bank Forest-Steppe. Scientific Reports of the National University of Life and Environmental Sciences of Ukraine, 22(3),136-146. https://doi.org/10.31548/dopovidi/3.2026.136
References
  1. Álvarez-Vázquez, P., Encina-Domínguez, J.A., Ventura-Rios, J., Hernández-Perez, A., Flores-Naveda, A., & Maldonado-Peralta, R. (2020). Productive performance of alfalfa (Medicago sativa L.) at different age of resprout in the spring season. Agro Productividad, 13(12), 113-118. doi: 10.32854/agrop.v13i12.1898
  2. Antoniv, S., Kolisnyk, S., & Zapruta, О. (2023). Alfalfa: History of the name origin, agroecological importance, basis for high-protein feed. Feeds and Feed Production, 96, 208-214. doi: 10.31073/kormovyrobnytsvo202396-19.
  3. Appiah, E.A., Balla-Kovács, A., Ocwa, A., Csajbók, J., & Kutasy, E. (2024). Enhancing alfalfa (Medicago sativa L.) productivity: Exploring the significance of potassium nutrition. Agronomy, 14(8), article number 1806. doi: 10.3390/agronomy14081806.
  4. Baidoo, M.M., & Islam, M.A. (2024). Potassium and harvest time interaction effect on alfalfa production and profitability. Agronomy Journal, 116(4), 1670-1687. doi: 10.1002/agj2.21575.
  5. Bélanger, G., Tremblay, G.F., Seguin, P., Lajeunesse, J., Bittman, S., & Hunt, D. (2020). Cutting management of alfalfa-based mixtures in contrasting agroclimatic regions. Agronomy Journal, 112(2), 1160-1175. doi: 10.1002/agj2.20142.
  6. Ben, Z., & Yang, H. (2025). Revisiting the effect of fertilizer application on biomass accumulation in alfalfa: Roles of fertilizer type, application rate, and environmental condition. Grass Research, 5, article number e028. doi: 10.48130/grares-0025-0025.
  7. Cholula, U., Andrade, M.A., & Solomon, J.K.Q. (2026). Effects of deficit irrigation on yield and nutritive value of alfalfa varieties in Northern Nevada’s semi-arid environment. Agricultural Water Management, 324, article number 110140. doi: 10.1016/j.agwat.2026.110140.
  8. Convention on Biological Diversity. (1992, June). Retrieved from https://zakon.rada.gov.ua/laws/show/995_030#Text.
  9. Desta, A.G. (2026). The role of alfalfa (Medicago sativa L.) in soil health and greenhouse gas mitigation in integrated crop-livestock systems: A review. Archives of Agronomy and Soil Science, 72(1), 1-16. doi: 10.1080/03650340.2025.2610090.
  10. DSTU 4674:2006. (2007). Hay. Specifications. Retrieved from https://online.budstandart.com/ua/catalog/doc-page?id_doc=75326.
  11. Herreid, J.S., Rand, T.A., Cockrell, D.M., Peairs, F.B., & Jabbour, R. (2024). On-farm harvest timing effects on alfalfa weevil across the Intermountain West region of the United States. Frontiers in Insect Science, 4, article number 1324044. doi: 10.3389/finsc.2024.1324044.
  12. Heuschele, D.J., Gamble, J., Vetsch, J.A., Shaeffer, C.C., Coulter, J.A., Kaiser, D.E., Lamb, J.A., Lamb, J.A.F.S., & Samac, D.A. (2023). Influence of potassium fertilization on alfalfa leaf and stem yield, forage quality, nutrient removal, and plant health. Agrosystems, Geosciences & Environment, 6(1), article number e20346. doi: 10.1002/agg2.20346.
  13. Jia, Z., Ou, C., Sun, S., Sun, M., Zhao, Y., Li, C., Zhao, S., Wang, J., Jia, S., & Mao, P. (2024). Optimizing drip irrigation managements to improve alfalfa seed yield in semiarid region. Agricultural Water Management, 297, article number 108830. doi: 10.1016/j.agwat.2024.108830.
  14. Li, X., An, J., & Hou, X. (2023). Effects of six consecutive years of irrigation and phosphorus fertilization on alfalfa yield. Plants, 12(11), article number 2227. doi: 10.3390/plants12112227.
  15. Liatukienė, A., Skuodienė, R., Muraškienė, M., Repšienė, R., Karčauskienė, D., Asakavičiūtė, R., Čekstere-Muižniece, G., & Osvalde, A. (2025). Impact of soil and climate on alfalfa growth and yield. Acta Agriculturae Scandinavica, Section B – Soil & Plant Science, 75(1), article number 2577422. doi: 10.1080/09064710.2025.2577422.
  16. Liu, J., Lu, F., Zhu, Y., Wu, H., Ahmad, I., Dong, G., Zhou, G., & Wu, Y. (2024). The effects of planting density and nitrogen application on the growth quality of alfalfa forage in saline soils. Agriculture, 14(2), article number 302. doi: 10.3390/agriculture14020302.
  17. Liu, L., Yu, H., Wang, Y., Liu, Y., Liu, H., Yang, Y., Jia, R., & Liu, Y. (2025). The positive effects of appropriate drought stress on alfalfa that saving water while ensuring quality and economic benefits. Plant Production Science, 28(2), 139-152. doi: 10.1080/1343943X.2025.2463511.
  18. Ma, H., Jiang, P., Zhang, X., Ma, W., Cai, Z., & Sun, Q. (2024). Effects of nitrogen fertilization combined with subsurface irrigation on alfalfa yield, water and nitrogen use efficiency, quality, and economic benefits. Frontiers in Plant Science, 15, article number 1339417. doi: 10.3389/fpls.2024.1339417.
  19. Ren, H., Ning, S., Yan, A., Zhao, Y., Li, N., & Huo, T. (2025). Response of alfalfa yield to rates and ratios of N, P, and K fertilizer in arid and semi-arid regions of China based on meta-analysis. Agronomy, 15(5), article number 1093. doi: 10.3390/agronomy15051093.
  20. Sulc, R.M., Arnold, A.M., Cassida, K.A., Albrecht, K.A., Hall, M.H., Min, D., Xu, X., Undersander, D.J., & van Santen, E. (2021). Changes in forage nutritive value of reduced-lignin alfalfa during regrowth. Crop Science, 61(2), 1478-1487. doi: 10.1002/csc2.20366.
  21. Tlahig, S., Neji, M., Atoui, A., Seddik, M., Dbara, M., Yahia, H., Nagaz, K., Najari, S., Khorchani, T., & Loumerem, M. (2024). Genetic and seasonal variation in forage quality of lucerne (Medicago sativa L.) for resilience to climate change in arid environments. Journal of Agriculture and Food Research, 15, article number 100986. doi: 10.1016/j.jafr.2024.100986.
  22. Wang, Y., Xie, J., Fan, F., Sun, Z., Yuan, F., Wang, Q., Yu, L., Liu, Y., Li, J., & Cui, L. (2024). Phosphorus fertilization enhanced overwintering, root system and forage yield of late-seeded alfalfa in sodic soils. Scientific Reports, 14, article number 18090. doi: 10.1038/s41598-024-67087-6.
  23. Zhang, L., et al. (2025). Optimizing phosphorus fertilization management is conducive to improving alfalfa yield and quality: A meta-analysis. Agriculture, 15(8), article number 797. doi: 10.3390/agriculture15080797.
  24. Zhao, J., Huang, R., Wang, X., Ma, C., Li, M., & Zhang, Q. (2023). Effects of combined nitrogen and phosphorus application on protein fractions and nonstructural carbohydrate of alfalfa. Frontiers in Plant Science, 14, article number 1124664. doi: 10.3389/fpls.2023.1124664.