Frost resistance of the columnar apple tree the method of direct freezing

O. Havryliuk, T. Kondratenko, B. Mazur
Abstract

Frost stress strongly affects the spatial distribution of plants. Among various weather hazards, frost causes the greatest economic losses in agriculture. Among various environmental hazards, it is frost that causes the greatest economic losses in agriculture. Although frost severely limits life forms and creates enormous economic losses, it has not been studied as thoroughly as other biotic or abiotic stresses. Frost resistance can be affected by many factors, including microclimate, soil condition, plant height, but they must be studied in a complex. The purpose of the research was to select frost-resistant cultivars and hybrids of the columnar apple tree under the conditions of the Forest Steppe of Ukraine. The research was carried out during 2021–2022 at the V.L. Symyrenko Department of Horticulture of the National University of Life and Environmental Sciences of Ukraine. The experimental basis for conducting research was the columnar apple tree plantations of the Training Laboratory «Fruit and Vegetable Garden». Frost resistance was determined during the period of deep rest by the method of direct freezing of one-year increments. Freezing was performed in the laboratory of plant physiology and microbiology of the National Academy of Sciences of Ukraine. In the researched plantations, when the test samples were frozen at temperatures of -25 and -30 °C during deep rest, different resistance of varieties and shoot parts to low temperatures was found. In all cultivars and hybrids of columnar type apple trees, the apical bud and generative buds were the most vulnerable, and the tissues of the middle and upper part of the shoot were the most resistant to frost. The damage index during general freezing was the lowest in the cultivars ‘Valuta’, ‘Sparta’, ‘Favoryt’, ‘Bilosnizhka’, as well as in the hybrids ‘9/110 Mykhailivske’, ‘11/15(2)’ and ‘9/78 Viktoriia’; it was the largest in the ‘Bolero’ cultivars. Freezing of experimental samples at temperatures of -25 and -30 °C did not result in frostbite, critical for plants. All studied cultivars and hybrids of columnar apple trees are recommended for further research and production. The obtained results will be interesting for both experienced gardeners and amateur gardeners who plan to grow columnar apple trees in their garden

Keywords

"Сo" gene, cultivars and hybrids of apple trees, fruit formations, influence of climate, shoot

Suggested citation
Havryliuk, O., Kondratenko, T., & Mazur, B. (2022). Frost resistance of the columnar apple tree the method of direct freezing. Scientific Reports of the National University of Life and Environmental Sciences of Ukraine, 18(6). https://doi.org/10.31548/dopovidi2022.06.004
References
  1. Charrier, G., Cochard, H., & Améglio, T. (2013a). Evaluation of the impact of frost resistances on potential altitudinal limit of trees. Tree Physiology, 33(9), 891-902. https://doi.org/10.1093/treephys/tpt062.
  2. Gansert, D. (2004). Treelines of the Japanese Alps—altitudinal distribution and species composition under contrasting winter climates. Flora - Morphology, Distribution, Functional Ecology of Plants, 199(2), 143-156. https://doi.org/10.1078/0367-2530-00143.
  3. Larcher, W. (2005). Climatic constraints drive the evolution of low temperature resistance in woody plants. Journal of Agricultural Meteorology, 61(4), 189-202. https://doi.org/10.2480/agrmet.61.189.
  4. Havryliuk, O., Kondratenko, T., Mazur, B., Tonkha, O., Andrusyk, Y., Kutovenko, V., Yakovlev, R., Kryvoshapka, V., Trokhymchuk, A., & Dmytrenko, Y. (2022b). Efficiency of productivity potential realization of different-age sites of a trunk of grades of columnar type apple-trees. Agronomy Research, 20(2), 241-260. https://doi.org/10.15159/AR.22.031.
  5. Snyder, R.L., & Melo-Abreu, J.P. (2005). Frost Protection: Fundamentals, Practice and Economics, Environment and Natural Resources Series. Rome: Food and Agriculture Organization of the United Nations, 223. Retrieved from https://www.fao.org/3/y7231e/y7231e.pdf.
  6. Vasylenko, O., Kondratenko, T., Havryliuk, O., Andrusyk, Y., Kutovenko, V., Dmytrenko, Y., Grevtseva, N., & Marchyshyna, Y. (2021). The study of the productivity potential of grape varieties according to the indicators of functional activity of leaves. Potravinarstvo Slovak Journal of Food Sciences, 15, 639-647. https://doi.org/10.5219/1638.
  7. Havryliuk, O., Kondratenko, T., Mazur, B., Kutovenko, V., Mazurenko, B., Voitsekhivska, O., & Dmytrenko, Y. (2022a). Morphophysiological peculiarities of productivity formation in columnar apple cultivars. Agronomy Research, 20(1), 148-160. https://doi.org/10.15159/AR.22.007.
  8. Fornari, B., Malvolti, M.E., Taurchini, D., Fineschi, S., Beritognolo, I., Maccaglia, E., & Cannata, F. (2001). Isozyme and organellar DNA analysis of genetic diversity in natural/naturalised European and Asiatic walnut (Juglans regia L.) populations. Acta Horticulturae, 544, 167-178. https://doi.org/10.17660/ActaHortic.2001.544.23.
  9. Manchester, S.R. (1989). Early history of the Juglandaceae. Plant Systematics and Evolution, 162, 231-250. https://doi.org/10.1007/BF00936919.
  10. Fady, B., Ducci, F., Aleta, N., Becquey, J., Vazquez, R.D., Lopez, F.F., Jay-Allemand, C., Lefèvre, F., Ninot, A., Panetsos, K., Paris, P., Pisanelli, A., & Rumpf, H. (2003). Walnut demonstrates strong genetic variability for adaptive and wood quality traits in a network of juvenile field tests across Europe. New Forests, 25, 211-225. https://doi.org/10.1023/A:1022939609548.
  11. Gavryliuk, O., Kondratenko, T., & Goncharuk, Ju. (2019). Features of formation of productivity of columnar apple-tree. Bulletin of Agricultural Science, 97(6), 27-34. https://doi.org/10.31073/agrovisnyk201906-04.
  12. Kollas, C., Koerner, C., & Randin, C.F. (2014). Spring frost and growing season length co-control the cold range limits of broadleaved trees. Journal of Biogeography, 41(4), 773-783. https://doi.org/10.1111/jbi.12238.
  13. Lang, G.A., Early, J.D., Martin, G.C., & Darnell, R.L. (1987). Endo-, para- and ecodormancy: physiological terminology and classification for dormancy research. HortScience, 22(3), 371-377.
  14. Charrier, G., Bonhomme, M., Lacointe, A., & Améglio, T. (2011). Are budburst dates, dormancy and cold acclimation in walnut trees (Juglans regia L.) under mainly genotypic or environmental control? International Journal of Biometeorology, 55, 763-774. https://doi.org/10.1007/s00484-011-0470-1.
  15. Bonhomme, M., Lacointe, A., & Rageau, R. (2013). Evidence for nonoccurrence of node-to-node or stem-to-bud transfer of chilling temperature signal for dormancy release. Advances in Horticultural Science, 27, 33-43.
  16. Basler, D., & Koerner, C. (2012). Photoperiod sensitivity of bud burst in 14 temperate forest tree species. Agricultural and Forest Meteorology, 165, 73-81. https://doi.org/10.1016/j.agrformet.2012.06.001.
  17. Laube, J., Sparks, T.H., Estrella, N., Hofler, J., Ankerst, D.P., & Menzel, A. (2014). Chilling outweighs photoperiod in preventing precocious spring development. Global Change Biology, 20(1), 170-182. https://doi.org/10.1111/gcb.12360.
  18. Lenz, A., Hoch, G., Vitasse, Y., & Koerner, C. (2013). European deciduous trees exhibit similar safety margins against damage by spring freeze events along elevational gradients. New Phytologist, 200(4), 1166-1175. https://doi.org/10.1111/nph.12452.
  19. Cittadini, E.D., de Ridder, N., Peri, P.L., & van Keulen, H. (2006). A method for assessing frost damage risk in sweet cherry orchards of South Patagonia. Agricultural and Forest Meteorology, 141(2-4), 235-243. https://doi.org/10.1016/j.agrformet.2006.10.011.
  20. Poirier, M., Lacointe, A., & Améglio, T. (2010). A semi-physiological model of cold hardening and dehardening in walnut stem. Tree Physiology, 30(12), 1555-1569. https://doi.org/10.1093/treephys/tpq087.
  21. Kawamura, K. (2010). A conceptual Framework for the study of modular responses to local environmental heterogeneity within the plant crown and a review of related concepts. Ecological Research, 25(4), 733-744. https://doi.org/10.1007/s11284-009-0688-0.
  22. Charrier, G., Poirier, M., Bonhomme, M., Lacointe, A., & Améglio, T. (2013b). Frost acclimation in different organs of walnut trees Juglans regia L.: how to link physiology and modelling? Tree Physiology, 33(11), 1229-1241. https://doi.org/10.1093/treephys/tpt090.
  23. Havryliuk, O., & Kondratenko, T. (2019). Specific of the Assimilation Surface of Columnar Apple-Tree. Agrobiodiversity for Improving Nutrition, Health and Life Quality, 3, 57-65. https://doi.org/10.15414/agrobiodiversity.2019.2585-8246.057-065.
  24. Havryliuk, O.S., Kondratenko, T.E., & Kytaiev, O.I. (2019). Of the functional state of plants of colonial cultivars of apple. Plant and Soil Science, 10(2), 70-80. https://doi.org/10.31548/agr2019.02.070.
  25. Winkel, T., Lhomme, J.P., Nina Laura, J.P., Alcon, C.M., del Castillo, C., & Rocheteau, A. (2009). Assessing the protective effect of vertically heterogeneous canopies against radiative frost: the case of quinoa on the Andean Altiplano. Agricultural and Forest Meteorology, 149(10), 1759-1768. https://doi.org/10.1016/j.agrformet.2009.06.005.
  26. Havryliuk, O.S., & Kondratenko, T.E. (2020). The intensity of photosynthesis of the surface of columnar apple-tree in the conditions of Kyiv. Scientific Reports of NULES of Ukraine, 2(84). https://doi.org/10.31548/dopovidi2020.02.013.
  27. Nobel, P.S. (1980). Morphology, nurse plants, and minimum apical temperatures for young Carnegiea gigantea. Botanical Gazette, 141(2), 188-191. https://doi.org/10.1086/337142.
  28. Rodrigo, J. (2000). Spring frosts in deciduous fruit trees—morphological damage and flower hardiness. Scientia Horticulturae, 85(3), 155-173. https://doi.org/10.1016/S0304-4238(99)00150-8.
  29. Sakai, A., & Larcher, W. (1987). Frost survival of plants. Responses and adaptation to freezing stress. Ecological Studies. Berlin: Springer Verlag, 62, 321. https://doi.org/10.1007/978-3-642-71745-1.
  30. Man, R., Colombo, S., Kayahara, G.J., Duckett, S., Velasquez, R., & Dang, Q.L. (2013). A case of extensive conifer needle browning in northwestern Ontario in 2012: winter drying or freezing damage? The Forestry Chronicle, 89, 675-680. https://doi.org/10.5558/tfc2013-120.
  31. Tranquillini, W. (1979). Physiological Ecology of the Alpine Timberline. Berlin: Springer-Verlag, 31. https://doi.org/10.1007/978-3-642-67107-4.
  32. Kalberer, S.R., Wisniewski, M., & Arora, R. (2006). Deacclimation and reacclimation of cold-hardy plants: current understanding and emerging concepts. Plant Science, 171(1), 3-16. https://doi.org/10.1016/j.plantsci.2006.02.013.
  33. Pagter, M., Hausman, J.F., & Arora, R. (2011). Deacclimation kinetics and carbohydrate changes in stem tissues of Hydrangea in response to an experimental warm spell. Plant Science, 180(1), 140-148. https://doi.org/10.1016/j.plantsci.2010.07.009.
  34. Saarinen, T., Lundell, R., & Hanninen, H. (2011). Recovery of photosynthetic capacity in Vaccinium vitis-idaea during mild spells in winter. Plant Ecology, 212, 1429-1440. https://doi.org/10.1007/s11258-011-9918-y.
  35. Lim, C.C., Krebs, S.L., & Arora, R. (2014). Cold hardiness increases with age in juvenile Rhododendron populations. Frontiers in Plant Science, 5, 542. https://doi.org/10.3389/fpls.2014.00542.
  36. Pramsohler, M., Hacker, J., & Neuner, G. (2012). Freezing pattern and frost killing temperature of apple (Malus domestica) wood under controlled conditions and in nature. Tree Physiology, 32(7), 819-828. https://doi.org/10.1093/treephys/tps046.
  37. Charrier, G., Pramsohler, M., Charra-Vaskou, K., Saudreau, M., Améglio, T., Neuner, G., et al. (2015). Ultrasonic emissions during ice nucleation and propagation in plant xylem. New Phytologist, 207(3), 570-578. https://doi.org/10.1111/nph.13361.
  38. Hacker, J., Ladinig, U., Wagner, J., & Neuner, G. (2011). Inflorescences of alpine cushion plants freeze autonomously and may survive subzero temperatures by supercooling. Plant Science, 180(1), 149-156. https://doi.org/10.1016/j.plantsci.2010.07.013.
  39. Kuprian, E., Briceño, V.F., Wagner, J., & Neuner, G. (2014). Ice barriers promote supercooling and prevent frost injury in reproductive buds, flowers and fruits of alpine dwarf shrubs throughout the summer. Environmental and Experimental Botany, 106, 4-12. https://doi.org/10.1016/j.envexpbot.2014.01.011.
  40. Pramsohler, M., & Neuner, G. (2013). Dehydration and osmotic adjustment in apple stem tissue during winter as it relates to the frost resistance of buds. Tree Physiology, 33(8), 807-816. https://doi.org/10.1093/treephys/tpt057.
  41. Rowland, L.J., Ogden, E.L., Takeda, F., Glenn, D.M., Ehlenfeldt, M.K., & Vinyard, B.T. (2013). Variation among highbush blueberry cultivars for frost tolerance of open flowers. HortScience, 48(6), 692-695. https://doi.org/10.21273/HORTSCI.48.6.692.
  42. Andergassen, S., & Bauer, H. (2002). Frost hardiness in the juvenile and adult life phase of ivy (Hedera helix L.). Plant Ecology, 161, 207-213. https://doi.org/10.1023/A:1020365422879.
  43. Lardon, A., & Triboi-Blondel, A.M. (1994). Freezing injury to ovules, pollen and seeds in winter rape. Journal of Experimental Botany, 45(8), 1177-1181. https://doi.org/10.1093/jxb/45.8.1177.
  44. Michaletz, S.T., & Johnson, E.A. (2006). Foliage influences forced convection heat transfer in conifer branches and buds. New Phytologist, 170(1), 87-98. https://doi.org/10.1111/j.1469-8137.2006.01661.x.
  45. Trokhymchuk, A.I., Makarova, D.H., & Kytaiev, O.I. (2012). Frost resistance potential of introduced varieties of apple (Malus domestica Borkh.) in the conditions of the Western forest-steppe of Ukraine. Scientific Reports of NULES of Ukraine, 180, 187-192.
  46. Torop, V.V., Hrokholskyi, V.V., Skrypchenko, N.V., & Moroz, P.A. (2005). Study of frost resistance of actinidia. Horticulture, 56, 213-221.
  47. Vasiuta, V.M., & Sereda, I.I. (2005). Peculiarities of frost resistance of apple trees in intensive orchards. Horticulture, 56, 189-195.
  48. Honcharuk, Yu.D. (2012). Winter hardiness of scab-immune varieties of apple (Malus domestica Borkh.). Scientific Bulletin of the NULES of Ukraine, 180, 192-199.
  49. Soloveva, M.A. (1988). Atlas of damage to fruit berry crops by frost, 48.
  50. Hrokholskyi, V.V. (2003). Methods of determining damage to fruit crops due to wintering, spring and autumn frosts. Monitoring of Fruit Crops, 127-135.
  51. Palahecha, R.M., Hrokholskyi, V.V., Kytaiev, O.I., & Fomichova, S.V. (2008). Frost resistance of deciduous magnolia shoot tissues. Introduction and Conservation of Plant Diversity. Bulletin of the Kyiv National University named after Taras Shevchenko, 8, 52-55.
  52. Adamenko, T.I., Kulbida, M.I., & Prokopenko, A.L. (2011). Agroclimatic guide for the territory of Ukraine. Kamianets-Podilskyi: PP Halahodza R.S., 108.
  53. Adamenko, T.I. (2014). Agroclimatic zoning of the territory of Ukraine taking into account climate change. Kyiv: TOV «RIA»BLITs, 18.
  54. Adamenko, T.I. (2008). Peculiarities of the development of spring processes in Ukraine during the period of global warming. Ahronom, 1, 10-11.
  55. DSTU 7863:2015. (2016). Soil quality. Determination of easily hydrolyzable nitrogen by the Kornfield method. Kyiv: State Enterprise "UkrNDNC", 5.
  56. DSTU 4115-2002. (2003). Soils. Determination of mobile compounds of phosphorus and potassium by the modified Chirikov method. Kyiv: State Committee of Ukraine for Technological Regulation and Consumer Policy, 9.
  57. Mezhenskyi, V.M. (2017). Basics of scientific research in horticulture. Calculations in Microsoft Excel: Tutorial. Kyiv: Lira-K Publishing House, 212.