Scientific Reports of the National University of Life and Environmental Sciences of Ukraine

The effect of testosterone on the bile acid and bile lipid composition in rats

І. Lupaina, А. Liashevych, Y. Reshetnik, S. Veselsky, М. Makarchuk
Download article
Abstract

The study of sexual differences in the regulation of exocrine liver function is one of the topical areas in hepatology. After all, the liver serves as a mediator in a number of systemic effects of sex hormones on the body and is a key organ of their metabolism. In particular, the correlation between the concentration of steroid hormones can determine the direction of physiological processes and their possible distortions. Methods: physiological, biochemical, methods of mathematical statistics. Cholesecretion increased in female rats under the influence of testosterone. Testosterone raised the concentration of taurocholic acid and at the end of the acute experiment the level of taurohenodeoxycholic and taurodeoxycholic acids significantly increased. By comparison, the content of glycocholates decreased significantly immediately after the administration of the hormone but at the end of the experiment, the content of glycocholic acid increased significantly. The level of free bile acids increased under the testosterone. Testosterone affected the bile lipid composition, in particular, it raised the concentrations of phospholipids, cholesterol and its ethers, while the content of free fatty acids decreased under the studied hormone. Testosterone when administered intraperitoneally to female rats significantly affects the concentration of conjugated and free cholate, which may indicate its involvement in metabolic transformations and transport of bile acids to the primary bile ducts. The studied hormone raised the concentration of phospholipids, cholesterol and its ethers, but reduced the content of free fatty acids in the liver secretion of the studied animals

Keywords

testosterone, bile, bile acids, lipids, liver

Suggested citation
Lupaina, І., Liashevych, А., Reshetnik, Y., Veselsky, S., & Makarchuk, М. (2021). The effect of testosterone on the bile acid and bile lipid composition in rats. Scientific Reports of the National University of Life and Environmental Sciences of Ukraine, 17(5),28-38. https://doi.org/10.31548/dopovidi2021.05.003
References
  1. Feysa, S.V. (2016). Thyroid pathologies and non-alcoholic fatty liver disease. Ukraine. Health of the Nation, 1-2(37-38), 198-202.
  2. Stinton, L.M., & Shaffer, E.A. (2012). Epidemiology of gallbladder disease: Cholelithiasis and cancer. Gut and Liver, 6(2), 172-187. https://doi.org/10.5009/gnl.2012.6.2.172.
  3. Burmak, Y.H., Kharchenko, V.V., & Yakubovskaya, I.A. (2013). Gallbladder cholesterosis: Etiology, pathogenesis, particular qualities. Problems of Environmental and Medical Genetics and Clinical Immunology, 1, 255-264.
  4. Chernukha, I.S., Reshetnik, E.M., Nuryshchenko, N.E., Liashevych, A.M., Kostenko, S.I., & Levchuk, T.M. (2017). Testosterone influence on the bile acids and lipids metabolites ratio in the bile in male and female rats. Journal "Phytotherapy", 2, 26-31.
  5. Kaminskyi, V.V., Sumenko, V.V., Kolomiichenko, T.V., Proshchenko, O.P., & Tatarchuk, T.F. (2016). Hormonal homeostasis in women with infertility and functional disorders of hepatobiliary system. Reproductive Endocrinology, 5(31), 8-11.
  6. Parkhomenko, L.K., Budreiko, O.A., Strashok, L.A., Voronenko, Y.V., & Marushko, Y.V. (2018). State of the hepatobiliary system in boys with hypoandrogenism. Modern Gastroenterology, 5(103), 19-24. https://doi.org/10.3978/MG-2018-5-19.
  7. Boyer, J.L. (2013). Bile formation and secretion. Comprehensive Physiology, 3(3), 1035-1078. https://doi.org/10.1002/cphy.c120027.
  8. Kelly, D.M., & Jones, T.H. (2013). Testosterone: A metabolic hormone in health and disease. Journal of Endocrinology, 3(217), 25-45. https://doi.org/10.1530/JOE-12-0455.
  9. Korpachev, V.V., Melnychenko, S.V., & Lukashova, R.G. (2015). Association of allele variants of receptor gene of androgens (by the number of CAG-repeats) with androgen dependent hormonal metabolic indices of the organism. Ukrainian Biochemical Journal, 2(87), 26-40.
  10. Nucci, R.A.B., Teodoro, A.C.S., Krause, N.W., Cordeiro, A., Borges, C.P., & Silva, L.M. (2017). Effects of testosterone administration on liver structure and function in aging rats. Aging Male, 20(2), 134-137. https://doi.org/10.1080/13685538.2017.1284779.
  11. Mintziori, G., Poulakos, P., Tsametis, C., Kotsa, K., & Goulis, D.G. (2017). Hypogonadism and non-alcoholic fatty liver disease. Minerva Endocrinologica, 42(2), 145-150. https://doi.org/10.23736/S0391-1977.16.02570-0.
  12. Borovets, O., Bened, V., Reshetnik, E., Kostenko, S., Lyashchenko, P., & Veselsky, S. (2016). Bile secretion liver function in the female rats at estrogen receptor tamoxifen blockade conditions. Scientific Bulletin of Lesya Ukrainka Eastern European National University, 7, 194-199.
  13. Veselsky, S.P., Lyashchenko, P.S., & Luk'janenko, Y.A. (1991). A method of determining bile acids in biological fluids. Author's Certificate No. 1624322.
  14. Veselsky, S.P., Lyashchenko, P.S., Kostenko, S.I., Horenko, Z.A., & Kurovska, L.F. (2001). Method of preparation of samples of bioridines to determine the content of substances of lipid nature. Ukrainian Patent 99031324.
  15. Korpachev, V.V., Lukashova, R.G., Melnytchenko, S.V., Gavrilyuk, O.A., & Serhiyenko, V.O. (2013). Genetic variability of CAG-repeats in androgen receptor gene in males with type 2 diabetes depending on different insulinemia levels. Endocrinology, 18(3), 20-27.
  16. Cai, Z., Xi, H., Pan, Y., Zhao, S., Zhang, Q., Dong, X., & Lu, C. (2015). Effect of testosterone deficiency on cholesterol metabolism in pigs fed a high-fat and high-cholesterol diet. Lipids in Health and Disease, 31, 123-456. https://doi.org/10.1186/s12944-015-0014-5.
  17. Chernuha, I.S., Reshetnik, Y.M., Liashevych, A.M., Kostenko, S.I., Lyashchenko, P.S., & Veselsky, S.P. (2018). Bile acids from bile of rats of different sexes under testosterone. Regulatory Mechanisms in Biosystems, 9(3), 396-400. https://doi.org/10.15421/021859.
  18. Scott, K.A., Melhorn, S.J., & Sakai, R.R. (2012). Effects of chronic social stress on obesity. Current Obesity Reports, 1(1), 16-25. https://doi.org/10.1007/s13679-011-0006-3.
  19. Moroz, O.F., Veselsky, S.P., Lyashchenko, T.P., Kostenko, S.I., & Reshetnik, E.M. (2009). Changes of lipid components ratio in the rat bile after applying bombesin neuropeptide. Ukrainian Biochemical Journal, 81(1), 52-58.